Application of Bacillus anthracis PCR to simulated clinical samples
نویسندگان
چکیده
منابع مشابه
Evaluation of PCR Systems for Field Screening of Bacillus anthracis
There is little published data on the performance of hand-portable polymerase chain reaction (PCR) systems that can be used by first responders to determine if a suspicious powder contains a potential biothreat agent. We evaluated 5 commercially available hand-portable PCR instruments for detection of Bacillus anthracis. We used a cost-effective, statistically based test plan to evaluate system...
متن کاملBacillus anthracis, virulence factors, PCR, and interpretation of results
Editorial Anthrax is a non-contagious infectious disease that affects a wide range of animal species, including humans, but especially sensitive are domestic and wild ruminants. The bacterial agent is Bacillus anthracis, whose main feature is to form spores that can survive in the environment for several decades. Anthrax, in susceptible animals, has a generally fatal outcome characterized by su...
متن کاملClinical Significance of Bacillus Species Other than Bacillus anthracis
Gram positive aerobic or facultatively anaerobic spore forming gram positive bacilli were first identified by Christian Gottfried Ehrenberg in 1885 and were later classified by Ferdinand Cohn in to a separate genus Bacillus [1]. Aerobic spore forming gram positive bacteria are commonly referred to as ASB’s and includes members of Bacillus spp which are basically saprophytes living as thermophil...
متن کاملReal-Time PCR Identification of Unique Bacillus anthracis Sequences.
Bacillus anthracis is a spore-forming, Gram-positive microorganism. It is a causative agent of anthrax, a highly infectious disease. It belongs to the "Bacillus cereus group", which contains other closely related species, including Bacillus cereus, Bacillus thuringiensis, Bacillus mycoides, Bacillus weihenstephanensis, and Bacillus pseudomycoides. B. anthracis naturally occurs in soil environme...
متن کاملDetection of Bacillus anthracis DNA by LightCycler PCR.
Anthrax is a zoonotic disease that is also well recognized as a potential agent of bioterrorism. Routine culture and biochemical testing methods are useful for the identification of Bacillus anthracis, but a definitive identification may take 24 to 48 h or longer and may require that specimens be referred to another laboratory. Virulent isolates of B. anthracis contain two plasmids (pX01 and pX...
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ژورنال
عنوان ژورنال: Clinical Microbiology and Infection
سال: 2003
ISSN: 1198-743X
DOI: 10.1046/j.1469-0691.2003.00736.x